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Signal transduction and targeted therapy, 2026/8/17

Fructose directly remodels the translocase of the outer membrane to impair oxidative phosphorylation in podocytes

PubMed ID: 42604929 DOI: 10.1038/s41392-026-02896-x

Wenyuan Wu, Wenhe Wang, ..., Lingdong Kong

ose with UV radiation. Podocytes were collected and centrifuged at 500 × g for 5 min. Podocytes were then resuspended in PBS, homogenized by sonication, and diluted to 2 mg/mL with PBS. The cell homogenate was combined with Azide-PEG3-biotin (100 μM; Jena Bioscience, Cat#CLK-AZ104P4), tris(2-carboxyethyl)phosphine hydrochloride (TCEP, 1 mM; Promega, Cat#VB1000), tris((1-benzyl-1H-1,2,3-triazol-4-yl)methyl)amine (TBTA, 100 μM; TargetMol, Cat#T7086) and CuSO 4 ·5H 2 O (1 mM; Sigma-Aldrich, Cat#1284

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