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Nature, 2026/9/2

Creating bottom-up RNA transfer vehicles from synthetic protein assemblies.

PubMed ID: 42686908 DOI: 10.1038/s41586-026-10952-3

Maren Kirstin Schuhmacher, Christoph Gruber, ..., Wolfgang Wurst

GFP expression following treatment was monitored after 24 h using an EVOS imaging device (Thermo Fisher Scientific).GFP expression following treatment was monitored after 24 h using an EVOS imaging device (Thermo Fisher Scientific).. In addition, in vitro-transcribed mTagBFP2 mRNA was produced as previously described, using N1-methylpseudouridine ( Jena BioScience) or unmodified uridine.. A549-IFN-GFP reporter cells were treated with STV-C8 containing mTagBFP2 mRNA or LNPs containing modified or unmodified mTagBFP2 mRNA to induce similar expression levels of mTagBFP2.A549-IFN-GFP reporter cells were treated with STV-C8 containing mTagBFP2 mRNA or LNPs containing modified or unmodified mTagBFP2 mRNA to induce similar expression levels of mTagBFP2.

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